|
gptkbp:instanceOf
|
gptkb:molecular_biology_technique
|
|
gptkbp:alternativeTo
|
gptkb:northern_blot
qPCR
|
|
gptkbp:canBe
|
qualitative
quantitative
one-step RT-PCR
two-step RT-PCR
|
|
gptkbp:canQuantify
|
mRNA levels
viral load
|
|
gptkbp:detects
|
gptkb:COVID-19
gptkb:hepatitis_C_virus
gptkb:influenza_virus
gptkb:HIV
|
|
gptkbp:developedBy
|
1980s
|
|
gptkbp:fullName
|
gptkb:reverse_transcription_polymerase_chain_reaction
|
|
gptkbp:inventedBy
|
gptkb:Kary_Mullis
|
|
gptkbp:limitation
|
requires high-quality RNA
requires specialized equipment
sensitive to contamination
|
|
gptkbp:output
|
amplified DNA
cDNA
|
|
gptkbp:relatedTo
|
gptkb:digital_PCR
gptkb:real-time_PCR
LAMP assay
|
|
gptkbp:requires
|
gptkb:reverse_transcriptase
gptkb:DNA_polymerase
gptkb:buffer_solution
nucleotides
primers
thermal cycler
template RNA
|
|
gptkbp:step
|
gptkb:polymerase_chain_reaction
reverse transcription
|
|
gptkbp:subspecies
|
PCR
nucleic acid amplification test
|
|
gptkbp:type
|
gptkb:gene
biological fluids
|
|
gptkbp:usedBy
|
public health agencies
research laboratories
clinical laboratories
biotechnology companies
|
|
gptkbp:usedFor
|
detecting RNA
diagnosing viral infections
quantifying gene expression
|
|
gptkbp:usedIn
|
COVID-19 testing
gene expression analysis
HIV detection
|
|
gptkbp:bfsParent
|
gptkb:t(8;21)_translocation
gptkb:ALK
gptkb:reverse_transcription_PCR
|
|
gptkbp:bfsLayer
|
6
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
RT-PCR
|