Statements (50)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:alternativeTo |
gptkb:northern_blot
qPCR |
| gptkbp:canBe |
qualitative
quantitative one-step RT-PCR two-step RT-PCR |
| gptkbp:canQuantify |
mRNA levels
viral load |
| gptkbp:detects |
gptkb:COVID-19
gptkb:hepatitis_C_virus gptkb:influenza_virus gptkb:HIV |
| gptkbp:developedBy |
1980s
|
| gptkbp:fullName |
gptkb:reverse_transcription_polymerase_chain_reaction
|
| https://www.w3.org/2000/01/rdf-schema#label |
RT-PCR
|
| gptkbp:inventedBy |
gptkb:Kary_Mullis
|
| gptkbp:limitation |
requires high-quality RNA
requires specialized equipment sensitive to contamination |
| gptkbp:output |
amplified DNA
cDNA |
| gptkbp:relatedTo |
gptkb:digital_PCR
gptkb:real-time_PCR LAMP assay |
| gptkbp:requires |
nucleotides
reverse transcriptase DNA polymerase buffer solution primers thermal cycler template RNA |
| gptkbp:step |
gptkb:polymerase_chain_reaction
reverse transcription |
| gptkbp:subspecies |
PCR
nucleic acid amplification test |
| gptkbp:type |
gptkb:gene
biological fluids |
| gptkbp:usedBy |
public health agencies
research laboratories clinical laboratories biotechnology companies |
| gptkbp:usedFor |
detecting RNA
diagnosing viral infections quantifying gene expression |
| gptkbp:usedIn |
COVID-19 testing
gene expression analysis HIV detection |
| gptkbp:bfsParent |
gptkb:Newcastle_disease
|
| gptkbp:bfsLayer |
4
|