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gptkbp:instanceOf
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molecular biology technique
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gptkbp:abbreviation
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qPCR
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gptkbp:advantage
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high sensitivity
quantitative results
rapid detection
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gptkbp:alsoKnownAs
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gptkb:quantitative_PCR
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gptkbp:application
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environmental monitoring
food safety testing
forensic analysis
clinical diagnostics
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gptkbp:canBe
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gptkb:two-step
one-step
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gptkbp:developedBy
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1990s
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gptkbp:enables
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real-time monitoring of PCR
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gptkbp:firstPublished
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1992
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https://www.w3.org/2000/01/rdf-schema#label
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real-time PCR
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gptkbp:inventedBy
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gptkb:Fred_C._F._Law
gptkb:Henry_C._F._F._Kwok
gptkb:Russell_Higuchi
gptkb:Timothy_D._H._Hsu
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gptkbp:limitation
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requires specialized equipment
can be affected by inhibitors
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gptkbp:monitors
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amplification of DNA during PCR
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gptkbp:output
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Ct value
amplification curve
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gptkbp:patent
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gptkb:Applied_Biosystems
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gptkbp:relatedTo
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gptkb:conventional_PCR
gptkb:digital_PCR
gptkb:reverse_transcription_PCR
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gptkbp:requires
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thermal cycler with fluorescence detection
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gptkbp:usedFor
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gene expression analysis
pathogen detection
quantification of nucleic acids
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gptkbp:uses
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fluorescent dyes
sequence-specific DNA probes
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gptkbp:bfsParent
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gptkb:RT-PCR
gptkb:polymerase_chain_reaction
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gptkbp:bfsLayer
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5
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