Statements (32)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:abbreviation |
gptkb:RT-PCR
|
| gptkbp:alternativeName |
gptkb:RT-PCR
gptkb:RT_PCR gptkb:reverse_transcriptase_PCR |
| gptkbp:application |
oncology
virology genetic research infectious disease diagnosis |
| gptkbp:detects |
messenger RNA
viral RNA |
| gptkbp:developedBy |
1980s
|
| gptkbp:enzyme |
reverse transcriptase
DNA polymerase |
| https://www.w3.org/2000/01/rdf-schema#label |
reverse transcription PCR
|
| gptkbp:inventedBy |
gptkb:Kary_Mullis
|
| gptkbp:numberOfStages |
reverse transcription of RNA to cDNA
|
| gptkbp:output |
amplified DNA
|
| gptkbp:processor |
gptkb:polymerase_chain_reaction
reverse transcription |
| gptkbp:relatedTo |
gptkb:quantitative_PCR
gptkb:real-time_PCR |
| gptkbp:requires |
nucleotides
buffer solution primers template RNA |
| gptkbp:step |
amplification of cDNA
|
| gptkbp:usedFor |
gene expression analysis
detecting RNA quantifying RNA |
| gptkbp:bfsParent |
gptkb:polymerase_chain_reaction
|
| gptkbp:bfsLayer |
5
|