reverse transcription polymerase chain reaction
GPTKB entity
Statements (39)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:abbreviation |
gptkb:RT-PCR
|
| gptkbp:analyzes |
gel electrophoresis
fluorescence detection |
| gptkbp:application |
cancer research
forensic science virology medical diagnostics |
| gptkbp:canBe |
one-step RT-PCR
two-step RT-PCR |
| gptkbp:category |
laboratory technique
nucleic acid amplification technique |
| gptkbp:detects |
gptkb:COVID-19
gptkb:influenza_virus gptkb:HIV |
| gptkbp:developedBy |
1980s
|
| https://www.w3.org/2000/01/rdf-schema#label |
reverse transcription polymerase chain reaction
|
| gptkbp:inventedBy |
gptkb:Kary_Mullis
|
| gptkbp:limitation |
requires high-quality RNA
sensitive to contamination can produce false positives |
| gptkbp:output |
amplified DNA
cDNA |
| gptkbp:regulates |
temperature cycling
|
| gptkbp:relatedTo |
gptkb:quantitative_PCR
gptkb:real-time_PCR |
| gptkbp:requires |
nucleotides
reverse transcriptase DNA polymerase primers template RNA |
| gptkbp:step |
gptkb:polymerase_chain_reaction
reverse transcription |
| gptkbp:usedFor |
gene expression analysis
detection of RNA quantification of RNA |
| gptkbp:usedIn |
COVID-19 testing
|
| gptkbp:bfsParent |
gptkb:RT-PCR
|
| gptkbp:bfsLayer |
5
|