reverse transcription polymerase chain reaction

URI: https://gptkb.org/entity/reverse_transcription_polymerase_chain_reaction
GPTKB entity
Statements (39)
Predicate Object
gptkbp:instanceOf gptkb:molecular_biology_technique
gptkbp:abbreviation gptkb:RT-PCR
gptkbp:analyzes gel electrophoresis
fluorescence detection
gptkbp:application cancer research
forensic science
virology
medical diagnostics
gptkbp:canBe one-step RT-PCR
two-step RT-PCR
gptkbp:category gptkb:laboratory_technique
nucleic acid amplification technique
gptkbp:detects gptkb:COVID-19
gptkb:influenza_virus
gptkb:HIV
gptkbp:developedBy 1980s
gptkbp:inventedBy gptkb:Kary_Mullis
gptkbp:limitation requires high-quality RNA
sensitive to contamination
can produce false positives
gptkbp:output amplified DNA
cDNA
gptkbp:regulates temperature cycling
gptkbp:relatedTo gptkb:quantitative_PCR
gptkb:real-time_PCR
gptkbp:requires gptkb:reverse_transcriptase
gptkb:DNA_polymerase
nucleotides
primers
template RNA
gptkbp:step gptkb:polymerase_chain_reaction
reverse transcription
gptkbp:usedFor gene expression analysis
detection of RNA
quantification of RNA
gptkbp:usedIn COVID-19 testing
gptkbp:bfsParent gptkb:RT-PCR
gptkbp:bfsLayer 7
https://www.w3.org/2000/01/rdf-schema#label reverse transcription polymerase chain reaction