northern blot

GPTKB entity

Statements (23)
Predicate Object
gptkbp:instanceOf molecular biology technique
gptkbp:application diagnosis of diseases
research in gene expression
study of mRNA levels
gptkbp:detects specific RNA sequences
gptkbp:developedBy gptkb:George_Stark
gptkb:James_Alwine
gptkb:David_Kemp
1977
https://www.w3.org/2000/01/rdf-schema#label northern blot
gptkbp:membraneType gptkb:nylon
nitrocellulose
gptkbp:relatedTo southern blot
western blot
gptkbp:sensorType DNA probe
RNA probe
gptkbp:step RNA separation by gel electrophoresis
hybridization with labeled probe
transfer to membrane
gptkbp:usedFor analysis of gene expression
detection of RNA
gptkbp:bfsParent gptkb:RT-PCR
gptkbp:bfsLayer 5