Statements (34)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:advantage |
absolute quantification without standard curve
higher precision than qPCR |
| gptkbp:alternativeTo |
gptkb:real-time_PCR
|
| gptkbp:application |
cancer research
environmental monitoring clinical diagnostics infectious disease detection |
| gptkbp:commercialUse |
gptkb:Bio-Rad
gptkb:Thermo_Fisher_Scientific |
| gptkbp:detects |
gptkb:gene
RNA (after reverse transcription) |
| gptkbp:developedBy |
1990s
|
| https://www.w3.org/2000/01/rdf-schema#label |
digital PCR
|
| gptkbp:limitation |
higher cost than qPCR
lower throughput than qPCR |
| gptkbp:partitionMethod |
gptkb:droplet_digital_PCR
chip-based digital PCR micro-well digital PCR |
| gptkbp:principle |
end-point PCR detection
partitioning sample into many individual PCR reactions |
| gptkbp:relatedTo |
gptkb:polymerase_chain_reaction
gptkb:quantitative_PCR |
| gptkbp:requires |
thermal cycler
fluorescent probes microfluidics or droplet generation |
| gptkbp:usedFor |
gene expression analysis
copy number variation analysis quantification of nucleic acids absolute quantification rare mutation detection |
| gptkbp:bfsParent |
gptkb:RT-PCR
gptkb:polymerase_chain_reaction |
| gptkbp:bfsLayer |
5
|