Statements (52)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:abbreviation |
PCR
|
| gptkbp:analyzes |
gel electrophoresis
|
| gptkbp:category |
gptkb:biotechnology
DNA technology laboratory technique |
| gptkbp:cycleNumber |
typically 20-40 cycles
|
| gptkbp:enables |
DNA fingerprinting
cloning of genes detection of pathogens quantification of DNA |
| gptkbp:firstPublished |
gptkb:Science_(journal)
1985 |
| https://www.w3.org/2000/01/rdf-schema#label |
polymerase chain reaction
|
| gptkbp:introducedIn |
1983
|
| gptkbp:inventedBy |
gptkb:Kary_Mullis
|
| gptkbp:limitation |
amplification errors
contamination risk inhibitors in samples primer design sensitivity |
| gptkbp:NobelPrizeYear |
Kary Mullis received Nobel Prize in Chemistry 1993
|
| gptkbp:notableEnzyme |
gptkb:Taq_polymerase
|
| gptkbp:patent |
gptkb:US4683202A
|
| gptkbp:product |
amplicon
|
| gptkbp:relatedTo |
gptkb:Sanger_sequencing
COVID-19 testing molecular diagnostics DNA replication genotyping |
| gptkbp:requires |
nucleotides
DNA polymerase DNA template buffer solution magnesium ions primers thermal cycler |
| gptkbp:step |
annealing
extension denaturation |
| gptkbp:temperatureCycle |
typically 3 steps per cycle
|
| gptkbp:usedFor |
amplification of DNA
|
| gptkbp:usedIn |
forensic science
genetic research medical diagnostics molecular cloning |
| gptkbp:variant |
gptkb:digital_PCR
gptkb:multiplex_PCR gptkb:nested_PCR gptkb:real-time_PCR gptkb:reverse_transcription_PCR |
| gptkbp:bfsParent |
gptkb:Kary_Mullis
|
| gptkbp:bfsLayer |
4
|