polymerase chain reaction

URI: https://gptkb.org/entity/polymerase_chain_reaction

GPTKB entity

Predicate	Object
gptkbp:instanceOf	gptkb:molecular_biology_technique
gptkbp:abbreviation	PCR
gptkbp:analyzes	gel electrophoresis
gptkbp:category	gptkb:biotechnology gptkb:laboratory_technique DNA technology
gptkbp:cycleNumber	typically 20-40 cycles
gptkbp:enables	DNA fingerprinting cloning of genes detection of pathogens quantification of DNA
gptkbp:firstPublished	gptkb:Science_(journal) 1985
gptkbp:introducedIn	1983
gptkbp:inventedBy	gptkb:Kary_Mullis
gptkbp:limitation	amplification errors contamination risk inhibitors in samples primer design sensitivity
gptkbp:NobelPrizeYear	Kary Mullis received Nobel Prize in Chemistry 1993
gptkbp:notableEnzyme	gptkb:Taq_polymerase
gptkbp:patent	gptkb:US4683202A
gptkbp:product	amplicon
gptkbp:relatedTo	gptkb:Sanger_sequencing COVID-19 testing molecular diagnostics DNA replication genotyping
gptkbp:requires	gptkb:DNA_polymerase gptkb:buffer_solution nucleotides DNA template magnesium ions primers thermal cycler
gptkbp:step	annealing extension denaturation
gptkbp:temperatureCycle	typically 3 steps per cycle
gptkbp:usedFor	amplification of DNA
gptkbp:usedIn	forensic science genetic research medical diagnostics molecular cloning
gptkbp:variant	gptkb:digital_PCR gptkb:multiplex_PCR gptkb:nested_PCR gptkb:real-time_PCR gptkb:reverse_transcription_PCR
gptkbp:bfsParent	gptkb:Kary_Mullis
gptkbp:bfsLayer	4
https://www.w3.org/2000/01/rdf-schema#label	polymerase chain reaction