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gptkbp:instanceOf
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gptkb:protein
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gptkbp:alternativeName
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gptkb:CRISPR-associated_protein_9
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gptkbp:component
|
gptkb:CRISPR-Cas_system
|
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gptkbp:discoveredBy
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gptkb:Emmanuelle_Charpentier
gptkb:Jennifer_Doudna
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gptkbp:foundIn
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gptkb:Streptococcus_pyogenes
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gptkbp:function
|
DNA cleavage
endonuclease
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|
https://www.w3.org/2000/01/rdf-schema#label
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Cas9 protein
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gptkbp:mechanismOfAction
|
forms complex with guide RNA
induces double-strand breaks
site-specific DNA targeting
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gptkbp:molecularWeight
|
~160 kDa
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gptkbp:orthologIn
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gptkb:Cpf1
gptkb:Cas12
gptkb:Cas13
|
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gptkbp:PDB
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gptkb:4CMP
gptkb:4UN3
gptkb:5F9R
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gptkbp:recognizedBy
|
PAM sequence
|
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gptkbp:requires
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gptkb:guide_RNA
|
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gptkbp:sequence
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gptkb:UniProt:_Q99ZW2
|
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gptkbp:structure
|
bilobed
|
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gptkbp:usedFor
|
drug discovery
synthetic biology
genetic engineering
base editing
gene therapy
prime editing
disease modeling
gene silencing
functional genomics
crop improvement
animal model creation
epigenome editing
gene activation
gene knock-in
gene knockout
gene repression
transcriptional regulation
targeted mutagenesis
gene tagging
screening genetic variants
studying gene function
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gptkbp:usedIn
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gptkb:biotechnology
gptkb:medicine
gptkb:CRISPR-Cas9_technology
agriculture
genome editing
|
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gptkbp:bfsParent
|
gptkb:CRISPR-Cas9_gene_editing_technology
gptkb:CRISPR
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gptkbp:bfsLayer
|
5
|