CRISPR-associated protein 9

URI: https://gptkb.org/entity/CRISPR-associated_protein_9
GPTKB entity
Statements (51)
Predicate Object
gptkbp:instanceOf gptkb:protein
gptkbp:alsoKnownAs gptkb:Cas9
gptkbp:clinicalTrialPhase gene editing therapies
gptkbp:component CRISPR adaptive immune system
gptkbp:discoveredBy gptkb:Emmanuelle_Charpentier
gptkb:Jennifer_Doudna
gptkbp:domain gptkb:PAM-interacting_domain
gptkb:HNH
gptkb:REC
gptkb:RuvC
gptkbp:EC_number 3.1.-.-
gptkbp:firstDescribed 2012
gptkbp:foundIn gptkb:Streptococcus_pyogenes
gptkbp:function genome editing
endonuclease
gptkbp:gene cas9
gptkbp:hasApplication synthetic biology
diagnostics
gene therapy
disease modeling
functional genomics
crop improvement
gptkbp:hasPatent gptkb:US20140068797A1
gptkb:US8697359B1
gptkb:WO2013176778A1
https://www.w3.org/2000/01/rdf-schema#label CRISPR-associated protein 9
gptkbp:limitation immune response
delivery challenges
off-target effects
gptkbp:mechanismOfAction creates double-strand breaks in DNA
gptkbp:molecularWeight ~160 kDa
gptkbp:orthologIn gptkb:Cpf1
gptkb:Cas12
gptkb:Cas13
gptkbp:PDB gptkb:4CMP
gptkb:5F9R
gptkbp:recognizedBy gptkb:guide_RNA
PAM sequence
gptkbp:requires gptkb:single_guide_RNA_(sgRNA)
Mg2+
gptkbp:structure multi-domain protein
gptkbp:targetSequence 20-nucleotide DNA sequence
gptkbp:UniProtID Q99ZW2
gptkbp:usedFor gptkb:agricultural_machinery
biomedical research
gene editing
genome engineering
gene knockout
gptkbp:usedIn gptkb:CRISPR-Cas9_system
gptkbp:bfsParent gptkb:Cas9_protein
gptkbp:bfsLayer 6