CRISPR-associated protein 9

GPTKB entity

Predicate	Object
gptkbp:instanceOf	gptkb:protein
gptkbp:alsoKnownAs	gptkb:Cas9
gptkbp:clinicalTrialPhase	gene editing therapies
gptkbp:component	CRISPR adaptive immune system
gptkbp:discoveredBy	gptkb:Emmanuelle_Charpentier gptkb:Jennifer_Doudna
gptkbp:domain	gptkb:PAM-interacting_domain gptkb:HNH gptkb:REC gptkb:RuvC
gptkbp:EC_number	3.1.-.-
gptkbp:firstDescribed	2012
gptkbp:foundIn	gptkb:Streptococcus_pyogenes
gptkbp:function	gptkb:endonuclease genome editing
gptkbp:gene	cas9
gptkbp:hasApplication	gptkb:gene_therapy synthetic biology diagnostics disease modeling functional genomics crop improvement
gptkbp:hasPatent	gptkb:US20140068797A1 gptkb:US8697359B1 gptkb:WO2013176778A1
gptkbp:limitation	gptkb:immune_response delivery challenges off-target effects
gptkbp:mechanismOfAction	creates double-strand breaks in DNA
gptkbp:molecularWeight	~160 kDa
gptkbp:orthologIn	gptkb:Cpf1 gptkb:Cas12 gptkb:Cas13
gptkbp:PDB	gptkb:4CMP gptkb:5F9R
gptkbp:recognizedBy	gptkb:guide_RNA PAM sequence
gptkbp:requires	gptkb:single_guide_RNA_(sgRNA) Mg2+
gptkbp:structure	multi-domain protein
gptkbp:targetSequence	20-nucleotide DNA sequence
gptkbp:UniProtID	Q99ZW2
gptkbp:usedFor	gptkb:agricultural_machinery biomedical research gene editing genome engineering gene knockout
gptkbp:usedIn	gptkb:CRISPR-Cas9_system
gptkbp:bfsParent	gptkb:Cas9 gptkb:Cas9_endonuclease gptkb:Cas9_protein
gptkbp:bfsLayer	7
https://www.w3.org/2000/01/rdf-schema#label	CRISPR-associated protein 9