FASTQ file format

GPTKB entity

Statements (68)
Predicate Object
gptkbp:instanceOf file format
gptkbp:canBeCompressedAs .fastq.gz
.fq.gz
gptkbp:commonIn genomics
bioinformatics
next-generation sequencing
gptkbp:contains quality values
raw sequence letters
sequence identifier
gptkbp:developedBy gptkb:Wellcome_Trust_Sanger_Institute
gptkbp:fileExtension .fastq
.fq
gptkbp:firstLineBeginsWith @
https://www.w3.org/2000/01/rdf-schema#label FASTQ file format
gptkbp:introducedIn 2000s
gptkbp:lineStructure 4 lines per sequence
gptkbp:mayInclude gptkb:UMIs_(Unique_Molecular_Identifiers)
barcodes
paired-end reads
single-end reads
gptkbp:qualityEncoding ASCII
Phred quality score
gptkbp:relatedTo gptkb:FASTA_file_format
gptkbp:specifies sequence quality for each base
gptkbp:standardizedBy yes
community practice
formal standards body
gptkbp:supportedBy gptkb:Picard
gptkb:galaxy
gptkb:Bowtie
gptkb:SAMtools
gptkb:BEDTools
gptkb:Cutadapt
gptkb:GATK
gptkb:HISAT2
gptkb:IGV
gptkb:MultiQC
gptkb:TopHat
gptkb:Trimmomatic
gptkb:UCSC_Genome_Browser
gptkb:Biopython
gptkb:Minimap2
gptkb:FASTQC
gptkb:Fastp
gptkb:NanoFilt
gptkb:Porechop
gptkb:SRA_Toolkit
gptkb:STAR_aligner
gptkb:SeqIO
gptkb:SeqKit
gptkb:Seqtk
gptkb:bcl2fastq
gptkb:seqtk
BWA
Cufflinks
gptkbp:thirdLineBeginsWith +
gptkbp:usedBy gptkb:BGI_sequencing
Illumina sequencing
Oxford Nanopore sequencing
gptkbp:usedFor storing nucleotide sequences
storing biological sequence data
storing quality scores
gptkbp:usedIn genome assembly
sequence alignment
variant calling
RNA-Seq analysis
gptkbp:bfsParent gptkb:Databricks_Runtime_for_Genomics
gptkbp:bfsLayer 7