UMIs (Unique Molecular Identifiers)
GPTKB entity
Statements (18)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:alternativeName |
molecular barcodes
|
| gptkbp:analyzes |
bioinformatics pipelines
|
| gptkbp:attachedTo |
DNA fragments
RNA fragments |
| gptkbp:composedOf |
short random nucleotide sequences
|
| gptkbp:enables |
identification of original molecules
removal of amplification bias |
| gptkbp:firstDescribed |
2012
|
| https://www.w3.org/2000/01/rdf-schema#label |
UMIs (Unique Molecular Identifiers)
|
| gptkbp:introducedIn |
library preparation
|
| gptkbp:length |
4-12 nucleotides
|
| gptkbp:usedFor |
improving quantification accuracy
reducing PCR duplicates |
| gptkbp:usedIn |
next-generation sequencing
single-cell RNA sequencing |
| gptkbp:bfsParent |
gptkb:FASTQ_file_format
|
| gptkbp:bfsLayer |
8
|