multiplex PCR

GPTKB entity

Statements (35)
Predicate Object
gptkbp:instanceOf molecular biology technique
gptkbp:advantage high throughput
cost effective
time saving
gptkbp:application genetic testing
gene expression analysis
forensic analysis
mutation analysis
pathogen detection
gptkbp:detects gel electrophoresis
capillary electrophoresis
fluorescent probes
gptkbp:developedBy 1988
gptkbp:field genetics
molecular biology
diagnostics
https://www.w3.org/2000/01/rdf-schema#label multiplex PCR
gptkbp:inventedBy Chamberlain et al.
gptkbp:limitation primer design complexity
primer-dimer formation
unequal amplification efficiency
gptkbp:relatedTo gptkb:conventional_PCR
gptkb:real-time_PCR
gptkb:reverse_transcription_PCR
gptkbp:requires gptkb:dNTPs
DNA polymerase
template DNA
buffer
multiple primer sets
gptkbp:usedFor simultaneous amplification of multiple DNA targets
gptkbp:usedIn environmental monitoring
food safety testing
clinical diagnostics
gptkbp:bfsParent gptkb:polymerase_chain_reaction
gptkbp:bfsLayer 5