Statements (56)
| Predicate | Object |
|---|---|
| gptkbp:instance_of |
gptkb:Biology
|
| gptkbp:application |
DNA sequencing
environmental testing medical diagnostics genetic research cloning agricultural biotechnology forensic analysis gene expression analysis |
| gptkbp:average_temperature |
50-80° C
|
| gptkbp:cloning_vector |
p GEM-T Easy
|
| gptkbp:commercial_sources |
various biotechnology companies
|
| gptkbp:commercially_available_forms |
liquid and lyophilized
|
| gptkbp:developed_by |
gptkb:Thermus_aquaticus
|
| gptkbp:discovered_by |
Mullis and Faloona
|
| gptkbp:error_rate |
approximately 1 in 10,000 nucleotides
|
| gptkbp:financial_stability |
high temperature stability
thermostable |
| gptkbp:function |
DNA synthesis
|
| https://www.w3.org/2000/01/rdf-schema#label |
Taq polymerase
|
| gptkbp:inactivation_temperature |
95° C
|
| gptkbp:initiated_by |
EDTA
|
| gptkbp:optimal_temperature |
75-80° C
|
| gptkbp:p_hlevel |
7.0-9.0
|
| gptkbp:produces |
amplified DNA
|
| gptkbp:reaction_buffer |
contains salts and p H stabilizers
|
| gptkbp:reaction_volume |
10-100 µ L
|
| gptkbp:requires |
magnesium ions
|
| gptkbp:response_time |
30 seconds to several minutes
|
| gptkbp:sensitivity |
high sensitivity to template DNA
|
| gptkbp:source |
gptkb:Thermus_aquaticus
|
| gptkbp:unit_definition |
one unit incorporates 10 nmol of d NTPs into acid-insoluble material in 30 minutes at 75° C
|
| gptkbp:used_in |
gptkb:Polymerase_chain_reaction_(PCR)
gptkb:biotechnology gptkb:Polymerase_Chain_Reaction_(PCR) metagenomics gene cloning multiplex PCR pathogen detection RNA analysis mutation detection DNA fingerprinting phylogenetic studies real-time PCR DNA barcoding digital PCR genotyping nested PCR quantitative PCR (q PCR) reverse transcription PCR (RT-PCR) SNP analysis high-fidelity PCR with modifications single-cell PCR |
| gptkbp:weight |
approximately 94 k Da
|
| gptkbp:bfsParent |
gptkb:Thermus_aquaticus
|
| gptkbp:bfsLayer |
7
|