Statements (18)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:firstDescribed |
1989
|
| https://www.w3.org/2000/01/rdf-schema#label |
nested PCR
|
| gptkbp:improves |
sensitivity of detection
|
| gptkbp:involves |
two sets of primers
two successive PCR reactions |
| gptkbp:reduces |
background noise
non-specific amplification |
| gptkbp:relatedTo |
gptkb:polymerase_chain_reaction
|
| gptkbp:requires |
template DNA
inner primers outer primers |
| gptkbp:used_in |
cloning
genetic research diagnostic applications |
| gptkbp:usedFor |
increasing specificity of DNA amplification
|
| gptkbp:bfsParent |
gptkb:polymerase_chain_reaction
|
| gptkbp:bfsLayer |
5
|