Statements (26)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
DNA sequencing method
|
| gptkbp:accuracy |
high
|
| gptkbp:alsoKnownAs |
chain-termination method
|
| gptkbp:detects |
gel electrophoresis
capillary electrophoresis |
| gptkbp:developedBy |
gptkb:Frederick_Sanger
|
| gptkbp:enables |
determination of nucleotide sequence
|
| gptkbp:fileNameLengthLimit |
up to 1000 base pairs
|
| https://www.w3.org/2000/01/rdf-schema#label |
Sanger sequencing
|
| gptkbp:introducedIn |
1977
|
| gptkbp:limitation |
expensive for large-scale sequencing
low throughput |
| gptkbp:NobelPrizeYear |
Frederick Sanger (1980, Chemistry)
|
| gptkbp:replacedBy |
next-generation sequencing
|
| gptkbp:significance |
first widely used DNA sequencing method
|
| gptkbp:usedFor |
DNA sequencing
|
| gptkbp:usedIn |
gptkb:Human_Genome_Project
|
| gptkbp:uses |
DNA polymerase
primers deoxynucleotides dideoxynucleotides template DNA |
| gptkbp:bfsParent |
gptkb:Frederick_Sanger
gptkb:gene gptkb:polymerase_chain_reaction |
| gptkbp:bfsLayer |
5
|