Statements (50)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
genome editing tool
|
| gptkbp:abbreviation |
gptkb:ZFN
|
| gptkbp:application |
animal genome modification
human gene therapy plant genome modification |
| gptkbp:cleavage |
dimerization of FokI domains required
|
| gptkbp:composedOf |
zinc finger DNA-binding domain
DNA-cleavage domain |
| gptkbp:contrastsWith |
gptkb:CRISPR-Cas9
gptkb:TALEN |
| gptkbp:deliveredBy |
gptkb:protein
gptkb:virus gptkb:gene plasmid |
| gptkbp:developedBy |
gptkb:Dana_Carroll
gptkb:Sangamo_BioSciences 1990s |
| gptkbp:DNA-cleavage_domain |
gptkb:FokI_nuclease
|
| gptkbp:firstPublished |
1996
|
| gptkbp:function |
induces double-strand breaks in DNA
|
| https://www.w3.org/2000/01/rdf-schema#label |
Zinc Finger Nuclease
|
| gptkbp:limitation |
cost
off-target effects design complexity |
| gptkbp:mechanismOfAction |
induces site-specific DNA double-strand breaks
|
| gptkbp:numberOfFingers |
typically 3 to 6 per ZFN
|
| gptkbp:patent |
gptkb:Sangamo_BioSciences
|
| gptkbp:recognizedBy |
zinc finger motifs bind 3 base pairs each
|
| gptkbp:repairMechanism |
gptkb:non-homologous_end_joining
gptkb:homology-directed_repair |
| gptkbp:target |
specific DNA sequences
|
| gptkbp:targetingMechanism |
engineered zinc finger proteins recognize specific DNA sequences
|
| gptkbp:usedFor |
genome editing
functional genomics crop improvement gene knockout gene correction gene insertion animal model generation HIV gene therapy beta-thalassemia gene editing gene correction in stem cells gene disruption sickle cell disease gene editing |
| gptkbp:usedIn |
gptkb:biotechnology
gptkb:medicine basic research clinical trials |
| gptkbp:bfsParent |
gptkb:ZFN
|
| gptkbp:bfsLayer |
6
|