Statements (23)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
gptkb:enzyme
|
| gptkbp:application |
genome engineering
molecular cloning |
| gptkbp:cleavage |
non-palindromic
9 nucleotides downstream on one strand, 13 nucleotides downstream on the other |
| gptkbp:cofactor |
Mg2+
|
| gptkbp:discoveredBy |
gptkb:Nobuyuki_Sugisaki
|
| gptkbp:discoveredIn |
1984
|
| gptkbp:EC_number |
3.1.21.4
|
| gptkbp:enzyme |
gptkb:Type_IIS_restriction_enzyme
|
| gptkbp:function |
DNA cleavage
|
| https://www.w3.org/2000/01/rdf-schema#label |
FokI nuclease
|
| gptkbp:mechanismOfAction |
dimerization required for DNA cleavage
|
| gptkbp:molecularWeight |
65 kDa
|
| gptkbp:organism |
gptkb:Flavobacterium_okeanokoites
|
| gptkbp:PDB |
gptkb:1FOK
|
| gptkbp:recognitionSequence |
5'-GGATG-3'
|
| gptkbp:UniProtID |
P13073
|
| gptkbp:usedIn |
gptkb:TALENs
genome editing zinc finger nucleases |
| gptkbp:bfsParent |
gptkb:Zinc_Finger_Nuclease
|
| gptkbp:bfsLayer |
7
|