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gptkbp:instanceOf
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genome editing technology
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gptkbp:advantage
|
high specificity
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gptkbp:application
|
animal genome editing
plant genome editing
human cell genome editing
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gptkbp:component
|
gptkb:TALE_DNA-binding_domain
FokI nuclease domain
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gptkbp:contrastsWith
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gptkb:CRISPR-Cas9
gptkb:ZFN
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gptkbp:deliveredBy
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gptkb:protein
gptkb:gene
plasmid
|
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gptkbp:developedBy
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gptkb:Joung_Lab
gptkb:Voytas_Lab
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gptkbp:firstDescribed
|
2010
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gptkbp:fullName
|
gptkb:Transcription_Activator-Like_Effector_Nuclease
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
TALEN
|
|
gptkbp:limitation
|
labor-intensive design
costly production
|
|
gptkbp:mechanismOfAction
|
DNA double-strand break induction
|
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gptkbp:patent
|
gptkb:Cellectis
gptkb:Sangamo_BioSciences
|
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gptkbp:recognitionCode
|
gptkb:TALE_repeat-variable_diresidue_(RVD)
|
|
gptkbp:relatedTo
|
gptkb:FokI_endonuclease
gptkb:TALE_proteins
|
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gptkbp:requires
|
dimerization for activity
|
|
gptkbp:target
|
specific DNA sequences
|
|
gptkbp:usedFor
|
genome editing
gene knockout
gene correction
gene insertion
targeted mutagenesis
|
|
gptkbp:usedIn
|
gptkb:agricultural_machinery
functional genomics
gene therapy research
|
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gptkbp:bfsParent
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gptkb:CRISPR
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gptkbp:bfsLayer
|
5
|