|
gptkbp:instanceOf
|
gptkb:genome_editing_technology
|
|
gptkbp:advantage
|
high specificity
|
|
gptkbp:application
|
animal genome editing
plant genome editing
human cell genome editing
|
|
gptkbp:component
|
gptkb:TALE_DNA-binding_domain
FokI nuclease domain
|
|
gptkbp:contrastsWith
|
gptkb:CRISPR-Cas9
gptkb:ZFN
|
|
gptkbp:deliveredBy
|
gptkb:protein
gptkb:gene
gptkb:plasmid
|
|
gptkbp:developedBy
|
gptkb:Joung_Lab
gptkb:Voytas_Lab
|
|
gptkbp:firstDescribed
|
2010
|
|
gptkbp:fullName
|
gptkb:Transcription_Activator-Like_Effector_Nuclease
|
|
gptkbp:limitation
|
labor-intensive design
costly production
|
|
gptkbp:mechanismOfAction
|
DNA double-strand break induction
|
|
gptkbp:patent
|
gptkb:Cellectis
gptkb:Sangamo_BioSciences
|
|
gptkbp:recognitionCode
|
gptkb:TALE_repeat-variable_diresidue_(RVD)
|
|
gptkbp:relatedTo
|
gptkb:FokI_endonuclease
gptkb:TALE_proteins
|
|
gptkbp:requires
|
dimerization for activity
|
|
gptkbp:target
|
specific DNA sequences
|
|
gptkbp:usedFor
|
genome editing
gene knockout
gene correction
gene insertion
targeted mutagenesis
|
|
gptkbp:usedIn
|
gptkb:agricultural_machinery
functional genomics
gene therapy research
|
|
gptkbp:bfsParent
|
gptkb:CRISPR/Cas9
gptkb:CRISPR
gptkb:CRISPR-Cas9
|
|
gptkbp:bfsLayer
|
6
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
TALEN
|