Statements (51)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:abbreviation |
PCR
|
| gptkbp:category |
laboratory technique
DNA amplification biotechnology method genetic engineering tool |
| gptkbp:cycleNumber |
typically 20-40 cycles
|
| gptkbp:effect |
exponential amplification of DNA
|
| gptkbp:enables |
DNA sequencing
gene expression analysis paternity testing genotyping mutation detection pathogen detection cloning of DNA |
| gptkbp:enzyme |
gptkb:Taq_polymerase
|
| gptkbp:firstPublished |
Science journal, 1985
|
| https://www.w3.org/2000/01/rdf-schema#label |
Polymerase chain reaction
|
| gptkbp:introducedIn |
1983
|
| gptkbp:inventedBy |
gptkb:Kary_Mullis
|
| gptkbp:NobelPrizeYear |
Kary Mullis received Nobel Prize in Chemistry 1993
|
| gptkbp:patent |
gptkb:US4683195A
|
| gptkbp:relatedTo |
gptkb:genetic_fingerprinting
COVID-19 testing molecular diagnostics DNA replication SARS-CoV-2 detection |
| gptkbp:requires |
nucleotides
DNA polymerase DNA template buffer solution primers thermal cycler |
| gptkbp:step |
annealing
extension denaturation |
| gptkbp:temperature |
typically 50°C to 98°C
|
| gptkbp:usedFor |
amplification of DNA
|
| gptkbp:usedIn |
gptkb:biotechnology
genetics molecular biology forensics medical diagnostics |
| gptkbp:variant |
gptkb:digital_PCR
gptkb:multiplex_PCR gptkb:nested_PCR gptkb:real-time_PCR gptkb:reverse_transcription_PCR |
| gptkbp:bfsParent |
gptkb:Nucleotide
gptkb:RT_PCR |
| gptkbp:bfsLayer |
7
|