Polymerase chain reaction

URI: https://gptkb.org/entity/Polymerase_chain_reaction
GPTKB entity
Statements (51)
Predicate Object
gptkbp:instanceOf gptkb:molecular_biology_technique
gptkbp:abbreviation PCR
gptkbp:category gptkb:laboratory_technique
gptkb:biotechnology_method
gptkb:genetic_engineering_tool
DNA amplification
gptkbp:cycleNumber typically 20-40 cycles
gptkbp:effect exponential amplification of DNA
gptkbp:enables DNA sequencing
gene expression analysis
paternity testing
genotyping
mutation detection
pathogen detection
cloning of DNA
gptkbp:enzyme gptkb:Taq_polymerase
gptkbp:firstPublished Science journal, 1985
gptkbp:introducedIn 1983
gptkbp:inventedBy gptkb:Kary_Mullis
gptkbp:NobelPrizeYear Kary Mullis received Nobel Prize in Chemistry 1993
gptkbp:patent gptkb:US4683195A
gptkbp:relatedTo gptkb:genetic_fingerprinting
COVID-19 testing
molecular diagnostics
DNA replication
SARS-CoV-2 detection
gptkbp:requires gptkb:DNA_polymerase
gptkb:buffer_solution
nucleotides
DNA template
primers
thermal cycler
gptkbp:step annealing
extension
denaturation
gptkbp:temperature typically 50°C to 98°C
gptkbp:usedFor amplification of DNA
gptkbp:usedIn gptkb:biotechnology
genetics
molecular biology
forensics
medical diagnostics
gptkbp:variant gptkb:digital_PCR
gptkb:multiplex_PCR
gptkb:nested_PCR
gptkb:real-time_PCR
gptkb:reverse_transcription_PCR
gptkbp:bfsParent gptkb:Nucleotide
gptkb:RT_PCR
gptkbp:bfsLayer 7
https://www.w3.org/2000/01/rdf-schema#label Polymerase chain reaction