Statements (33)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
Molecular biology technique
|
| gptkbp:advantage |
Specificity
High sensitivity |
| gptkbp:alternativeName |
gptkb:RT-PCR
Reverse transcriptase PCR |
| gptkbp:detects |
RNA viruses
Gene transcripts |
| gptkbp:developedBy |
1980s
|
| gptkbp:fullName |
Reverse Transcription Polymerase Chain Reaction
|
| https://www.w3.org/2000/01/rdf-schema#label |
RT PCR
|
| gptkbp:inventedBy |
gptkb:Kary_Mullis
|
| gptkbp:limitation |
Requires specialized equipment
Sensitive to contamination |
| gptkbp:output |
cDNA
Amplified DNA |
| gptkbp:relatedTo |
PCR
qPCR |
| gptkbp:requires |
DNA polymerase
Reverse transcriptase enzyme |
| gptkbp:step |
gptkb:Polymerase_chain_reaction
Reverse transcription |
| gptkbp:type |
gptkb:Blood
Tissue Swab |
| gptkbp:usedFor |
Detection of RNA
Gene expression analysis Viral detection |
| gptkbp:usedIn |
COVID-19 testing
Genetics Virology Medical diagnostics |
| gptkbp:bfsParent |
gptkb:reverse_transcription_PCR
|
| gptkbp:bfsLayer |
6
|