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gptkbp:instanceOf
|
gptkb:protein
gptkb:CRISPR-Cas_system
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|
gptkbp:affiliatedWith
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gptkb:Type_V_CRISPR_system
Class 2 CRISPR system
|
|
gptkbp:alsoKnownAs
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gptkb:Cpf1
|
|
gptkbp:cleavage
|
double-stranded DNA
staggered cut
|
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gptkbp:compatibleWith
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gptkb:tracrRNA
|
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gptkbp:discoveredBy
|
gptkb:Feng_Zhang
|
|
gptkbp:discoveredIn
|
2015
|
|
gptkbp:foundIn
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gptkb:Bacteria
|
|
gptkbp:function
|
genome editing
DNA cleavage
|
|
gptkbp:hasApplication
|
gptkb:DETECTR_diagnostic_platform
gptkb:SHERLOCK_diagnostic_platform
|
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gptkbp:hasCollateralActivity
|
single-stranded DNA cleavage
|
|
gptkbp:hasPatent
|
gptkb:US20160145536A1
gptkb:WO2016186946A1
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
Cas12a
|
|
gptkbp:mechanismOfAction
|
RNA-guided DNA endonuclease
|
|
gptkbp:orthologIn
|
gptkb:AsCas12a
gptkb:FnCas12a
gptkb:LbCas12a
gptkb:MbCas12a
|
|
gptkbp:PDB
|
gptkb:5B43
gptkb:5ID6
gptkb:5MGA
gptkb:5XUS
5NFV
|
|
gptkbp:produces
|
sticky ends
|
|
gptkbp:recognizesPAM
|
TTTV
|
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gptkbp:relatedTo
|
gptkb:Cas9
|
|
gptkbp:requires
|
gptkb:crRNA
|
|
gptkbp:targetSequence
|
gptkb:gene
|
|
gptkbp:usedFor
|
gene activation
gene knockout
gene repression
nucleic acid detection
targeted genome modification
|
|
gptkbp:usedIn
|
diagnostics
gene editing
genome engineering
point-of-care diagnostics
COVID-19 diagnostics
viral detection
plant genome editing
bacterial genome editing
mammalian genome editing
|
|
gptkbp:bfsParent
|
gptkb:CRISPR-Cas12_enzyme
gptkb:Cas12_protein
gptkb:CRISPR-Cas12
|
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gptkbp:bfsLayer
|
6
|