Statements (49)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
gptkb:CRISPR-Cas_system
|
| gptkbp:advantage |
broad PAM compatibility
simpler guide RNA requirements smaller size than SpCas9 staggered DNA cuts |
| gptkbp:affiliatedWith |
gptkb:Type_V_CRISPR_system
Class 2 CRISPR system |
| gptkbp:alsoKnownAs |
gptkb:Cpf1
|
| gptkbp:cleavage |
double-stranded DNA
staggered cut |
| gptkbp:collateralActivityType |
non-specific single-stranded DNA cleavage
|
| gptkbp:compatibleWith |
gptkb:tracrRNA
|
| gptkbp:discoveredIn |
2015
|
| gptkbp:fullName |
gptkb:Lachnospiraceae_bacterium_Cas12a
|
| gptkbp:function |
gptkb:DNA_endonuclease
Genome editing |
| gptkbp:hasCollateralActivity |
yes
|
| https://www.w3.org/2000/01/rdf-schema#label |
LbCas12a
|
| gptkbp:limitation |
off-target effects
temperature sensitivity PAM sequence requirement |
| gptkbp:origin |
gptkb:Lachnospiraceae_bacterium_ND2006
|
| gptkbp:recognizesPAM |
TTTV (where V is A, C, or G)
|
| gptkbp:relatedTo |
gptkb:AsCas12a
gptkb:FnCas12a gptkb:SpCas9 |
| gptkbp:requires |
gptkb:crRNA
|
| gptkbp:usedBy |
molecular biologists
biotechnology researchers diagnostic developers |
| gptkbp:usedFor |
gptkb:DETECTR_diagnostic_platform
gptkb:SHERLOCK_diagnostic_platform |
| gptkbp:usedIn |
diagnostics
in vitro diagnostics gene editing genome engineering base editing point-of-care diagnostics COVID-19 diagnostics nucleic acid detection viral detection animal genome editing plant genome editing SARS-CoV-2 detection human cell genome editing bacterial detection multiplexed nucleic acid detection |
| gptkbp:bfsParent |
gptkb:Cas12a
|
| gptkbp:bfsLayer |
7
|