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gptkbp:instanceOf
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genome editing tool
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gptkbp:abbreviation
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gptkb:ZFNs
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gptkbp:alternativeTo
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gptkb:CRISPR-Cas9
gptkb:TALENs
meganucleases
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gptkbp:application
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animal genome editing
plant genome editing
human gene therapy
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gptkbp:composedOf
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FokI DNA-cleavage domain
zinc finger DNA-binding domain
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gptkbp:deliveredBy
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gptkb:protein
gptkb:virus
gptkb:gene
plasmid
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gptkbp:developedBy
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gptkb:Dana_Carroll
gptkb:Sangamo_BioSciences
1990s
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gptkbp:firstPublished
|
1996
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gptkbp:function
|
create double-strand breaks in DNA
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https://www.w3.org/2000/01/rdf-schema#label
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Zinc finger nucleases
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gptkbp:limitation
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off-target effects
cytotoxicity
difficult design process
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gptkbp:mechanismOfAction
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bind to specific DNA sequences
induce DNA double-strand breaks
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gptkbp:modularDesign
|
customizable DNA-binding domain
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gptkbp:patent
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gptkb:Sangamo_BioSciences
|
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gptkbp:recognitionSiteLength
|
18-36 base pairs
|
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gptkbp:relatedTo
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gptkb:CRISPR-Cas9
gptkb:TALENs
|
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gptkbp:repairPathways
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gptkb:non-homologous_end_joining
gptkb:homology-directed_repair
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gptkbp:requires
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dimerization for activity
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gptkbp:target
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specific DNA sequences
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gptkbp:usedFor
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genome editing
disease modeling
functional genomics
cell line engineering
crop improvement
gene knockout
gene correction
gene insertion
gene disruption
transgenic animal creation
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gptkbp:usedIn
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gptkb:biotechnology
gptkb:medicine
basic research
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gptkbp:bfsParent
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gptkb:TALENs
gptkb:Gene_Therapy
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gptkbp:bfsLayer
|
7
|