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gptkbp:instanceOf
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genome editing technology
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gptkbp:advantage
|
high specificity
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gptkbp:alternativeTo
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gptkb:CRISPR-Cas9
gptkb:Zinc_finger_nucleases
|
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gptkbp:application
|
human cell genome editing
animal genome engineering
plant genome engineering
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gptkbp:composedOf
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FokI nuclease domain
DNA-binding domain
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gptkbp:firstDescribed
|
2010
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gptkbp:fullName
|
gptkb:Transcription_Activator-Like_Effector_Nucleases
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|
https://www.w3.org/2000/01/rdf-schema#label
|
TALENs
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gptkbp:limitation
|
labor-intensive design
delivery challenges in vivo
relatively high cost
|
|
gptkbp:mechanismOfAction
|
induces double-strand breaks in DNA
|
|
gptkbp:origin
|
derived from transcription activator-like effectors (TALEs) of Xanthomonas bacteria
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|
gptkbp:patent
|
gptkb:Sangamo_BioSciences
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gptkbp:recognizedBy
|
specific DNA sequences
|
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gptkbp:relatedTo
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gptkb:FokI_endonuclease
gptkb:TALE_proteins
|
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gptkbp:usedFor
|
genome editing
gene knock-in
gene knockout
targeted mutagenesis
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|
gptkbp:bfsParent
|
gptkb:J._Keith_Joung
gptkb:TALEN_genome_editing
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gptkbp:bfsLayer
|
6
|