Statements (48)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
genome editing technology
|
| gptkbp:alternativeTo |
gptkb:CRISPR-Cas9
gptkb:TALENs |
| gptkbp:component |
FokI nuclease domain
zinc finger DNA-binding domain |
| gptkbp:deliveredBy |
gptkb:virus
gptkb:gene plasmid |
| gptkbp:developedBy |
1990s
|
| gptkbp:firstDescribed |
gptkb:Dana_Carroll
gptkb:J._Keith_Joung 1996 Carl O. Pabo |
| gptkbp:fullName |
Zinc Finger Nucleases
|
| https://www.w3.org/2000/01/rdf-schema#label |
ZFNs
|
| gptkbp:limitation |
off-target effects
complex protein engineering |
| gptkbp:mechanismOfAction |
DNA double-strand break induction
|
| gptkbp:patent |
gptkb:Sangamo_Therapeutics
|
| gptkbp:requires |
custom protein engineering
|
| gptkbp:targetRecognition |
DNA sequence-specific
|
| gptkbp:usedFor |
gptkb:biotechnology
biomedical research genome editing therapeutic genome editing gene therapy crop improvement gene knockout gene correction functional genomics studies targeted mutagenesis gene disruption knock-in mutations transgenic animal creation disease model creation functional gene analysis transgenic plant creation |
| gptkbp:usedIn |
clinical trials
animal genome editing plant genome editing sickle cell disease research human cell genome editing HIV therapy research beta-thalassemia research |
| gptkbp:bfsParent |
gptkb:ARCUS_genome_editing_technology
gptkb:Zinc_finger_nucleases gptkb:TALEN_gene_editing |
| gptkbp:bfsLayer |
8
|