FokI endonuclease

GPTKB entity

Statements (23)
Predicate Object
gptkbp:instanceOf gptkb:enzyme
gptkbp:cleavage sticky ends
non-palindromic
9 nucleotides downstream on one strand, 13 nucleotides downstream on the other
gptkbp:cofactor Mg2+
gptkbp:discoveredBy gptkb:Nobuyuki_Sugisaki
gptkbp:discoveredIn 1984
gptkbp:EC_number 3.1.21.4
gptkbp:enzyme gptkb:Type_IIS_restriction_enzyme
gptkbp:gene fokIR
https://www.w3.org/2000/01/rdf-schema#label FokI endonuclease
gptkbp:mechanismOfAction binds DNA as a monomer, cleaves as a dimer
gptkbp:molecularWeight 65 kDa
gptkbp:organism gptkb:Flavobacterium_okeanokoites
gptkbp:PDB gptkb:1FOK
gptkbp:recognitionSequence 5'-GGATG-3'
gptkbp:structure dimeric
gptkbp:usedIn gptkb:TALENs
zinc finger nucleases
gptkbp:uses genome editing
molecular biology research
gptkbp:bfsParent gptkb:TALEN
gptkbp:bfsLayer 6