Statements (23)
Predicate | Object |
---|---|
gptkbp:instanceOf |
gptkb:enzyme
|
gptkbp:cleavage |
sticky ends
non-palindromic 9 nucleotides downstream on one strand, 13 nucleotides downstream on the other |
gptkbp:cofactor |
Mg2+
|
gptkbp:discoveredBy |
gptkb:Nobuyuki_Sugisaki
|
gptkbp:discoveredIn |
1984
|
gptkbp:EC_number |
3.1.21.4
|
gptkbp:enzyme |
gptkb:Type_IIS_restriction_enzyme
|
gptkbp:gene |
fokIR
|
https://www.w3.org/2000/01/rdf-schema#label |
FokI endonuclease
|
gptkbp:mechanismOfAction |
binds DNA as a monomer, cleaves as a dimer
|
gptkbp:molecularWeight |
65 kDa
|
gptkbp:organism |
gptkb:Flavobacterium_okeanokoites
|
gptkbp:PDB |
gptkb:1FOK
|
gptkbp:recognitionSequence |
5'-GGATG-3'
|
gptkbp:structure |
dimeric
|
gptkbp:usedIn |
gptkb:TALENs
zinc finger nucleases |
gptkbp:uses |
genome editing
molecular biology research |
gptkbp:bfsParent |
gptkb:TALEN
|
gptkbp:bfsLayer |
6
|