Statements (48)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:abbreviation |
PCR
|
| gptkbp:category |
gptkb:biotechnology
medical research forensic science genetic testing DNA technology laboratory technique |
| gptkbp:cycleNumber |
typically 20-40 cycles
|
| gptkbp:enables |
gptkb:genetic_fingerprinting
DNA sequencing gene expression analysis detection of pathogens cloning of DNA |
| gptkbp:enzyme |
gptkb:Taq_polymerase
|
| https://www.w3.org/2000/01/rdf-schema#label |
Polymerase Chain Reaction
|
| gptkbp:introducedIn |
1983
|
| gptkbp:inventedBy |
gptkb:Kary_Mullis
|
| gptkbp:limitation |
contamination risk
primer design sensitivity enzyme fidelity |
| gptkbp:NobelPrizeYear |
Kary Mullis received Nobel Prize in Chemistry 1993
|
| gptkbp:product |
amplicon
|
| gptkbp:relatedTo |
molecular diagnostics
genetic engineering DNA replication |
| gptkbp:requires |
nucleotides
DNA polymerase DNA template buffer solution primers |
| gptkbp:step |
annealing
extension denaturation |
| gptkbp:temperatureCycle |
typically 3 steps per cycle
|
| gptkbp:usedFor |
amplification of DNA
|
| gptkbp:usedIn |
gptkb:biotechnology
genetics molecular biology forensics medical diagnostics |
| gptkbp:variant |
gptkb:digital_PCR
gptkb:multiplex_PCR gptkb:nested_PCR gptkb:real-time_PCR gptkb:reverse_transcription_PCR |
| gptkbp:bfsParent |
gptkb:Fluorescence_In_Situ_Hybridization
|
| gptkbp:bfsLayer |
7
|