Polymerase Chain Reaction

URI: https://gptkb.org/entity/Polymerase_Chain_Reaction

GPTKB entity

Predicate	Object
gptkbp:instanceOf	gptkb:molecular_biology_technique
gptkbp:abbreviation	PCR
gptkbp:category	gptkb:biotechnology gptkb:laboratory_technique medical research forensic science genetic testing DNA technology
gptkbp:cycleNumber	typically 20-40 cycles
gptkbp:enables	gptkb:genetic_fingerprinting DNA sequencing gene expression analysis detection of pathogens cloning of DNA
gptkbp:enzyme	gptkb:Taq_polymerase
gptkbp:introducedIn	1983
gptkbp:inventedBy	gptkb:Kary_Mullis
gptkbp:limitation	contamination risk primer design sensitivity enzyme fidelity
gptkbp:NobelPrizeYear	Kary Mullis received Nobel Prize in Chemistry 1993
gptkbp:product	amplicon
gptkbp:relatedTo	molecular diagnostics genetic engineering DNA replication
gptkbp:requires	gptkb:DNA_polymerase gptkb:buffer_solution nucleotides DNA template primers
gptkbp:step	annealing extension denaturation
gptkbp:temperatureCycle	typically 3 steps per cycle
gptkbp:usedFor	amplification of DNA
gptkbp:usedIn	gptkb:biotechnology genetics molecular biology forensics medical diagnostics
gptkbp:variant	gptkb:digital_PCR gptkb:multiplex_PCR gptkb:nested_PCR gptkb:real-time_PCR gptkb:reverse_transcription_PCR
gptkbp:bfsParent	gptkb:Fluorescence_In_Situ_Hybridization
gptkbp:bfsLayer	8
https://www.w3.org/2000/01/rdf-schema#label	Polymerase Chain Reaction