Fluorescence In Situ Hybridization
GPTKB entity
Statements (49)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
Molecular Biology Technique
|
| gptkbp:abbreviation |
gptkb:FISH
|
| gptkbp:alternativeTo |
gptkb:Southern_blot
Next-generation sequencing qPCR |
| gptkbp:appliesTo |
Cell preparations
Chromosome spreads Tissue sections |
| gptkbp:combines |
Immunohistochemistry
Flow cytometry Confocal microscopy |
| gptkbp:detects |
Microbial pathogens
Nucleic acids Chromosomal abnormalities Gene amplifications Gene deletions Gene translocations |
| gptkbp:developedBy |
1980s
|
| gptkbp:firstPublished |
1969
|
| https://www.w3.org/2000/01/rdf-schema#label |
Fluorescence In Situ Hybridization
|
| gptkbp:inventedBy |
gptkb:Joseph_G._Gall
gptkb:Mary-Lou_Pardue |
| gptkbp:limitation |
Time-consuming
May not detect small mutations Requires specialized equipment Resolution limited by probe size |
| gptkbp:relatedTo |
gptkb:Comparative_Genomic_Hybridization
gptkb:Polymerase_Chain_Reaction Immunofluorescence |
| gptkbp:requires |
Washing steps
Denaturation of DNA Hybridization of probe Microscopy analysis |
| gptkbp:usedFor |
Cancer diagnosis
Chromosome mapping Detection of specific DNA sequences Detection of specific RNA sequences Diagnosis of genetic disorders Gene expression analysis Microbial identification |
| gptkbp:usedIn |
Microbiology
Oncology Pathology Cytogenetics Prenatal diagnosis |
| gptkbp:uses |
Fluorescent probes
|
| gptkbp:visualizes |
Fluorescence microscopy
|
| gptkbp:bfsParent |
gptkb:FISH
|
| gptkbp:bfsLayer |
6
|