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gptkbp:instanceOf
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CRISPR-associated system
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gptkbp:alternativeName
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gptkb:Cpf1_system
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gptkbp:application
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gptkb:DETECTR_diagnostic_platform
gptkb:SHERLOCK_diagnostic_platform
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gptkbp:class
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gptkb:Type_V_CRISPR_system
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gptkbp:cleavage
|
staggered double-stranded DNA breaks
|
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gptkbp:compatibleWith
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gptkb:tracrRNA
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gptkbp:component
|
CRISPR-Cas adaptive immune system
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gptkbp:differenceFromCas9
|
requires shorter guide RNA
creates sticky ends
recognizes different PAM
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gptkbp:discoveredBy
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gptkb:Feng_Zhang_lab
|
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gptkbp:discoveredIn
|
2015
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gptkbp:foundIn
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gptkb:Bacteria
archaea
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gptkbp:function
|
genome editing
DNA interference
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gptkbp:guideRNAType
|
crRNA only
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gptkbp:hasProtein
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gptkb:Cas12
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
Cas12 system
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gptkbp:mechanismOfAction
|
RNA-guided endonuclease activity
|
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gptkbp:notableFor
|
gptkb:Cas12a
gptkb:Cas12b
gptkb:Cas12c
gptkb:Cas12e
gptkb:Cas12f
|
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gptkbp:PAMSequence
|
TTTV (where V is A, C, or G)
|
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gptkbp:property
|
collateral single-stranded DNA cleavage activity
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gptkbp:recognizedBy
|
PAM sequence
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gptkbp:relatedTo
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gptkb:Type_V_CRISPR_system
gptkb:CRISPR-Cas9_system
Cas13 system
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gptkbp:target
|
double-stranded DNA
|
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gptkbp:usedFor
|
viral detection
animal genome editing
plant genome editing
bacterial detection
human genome editing
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gptkbp:usedIn
|
biosensing
molecular diagnostics
diagnostics
genome engineering
base editing
point-of-care testing
epigenome editing
gene knock-in
gene knock-out
transcriptional regulation
antiviral defense
antibacterial defense
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gptkbp:bfsParent
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gptkb:Type_V_CRISPR-Cas_system
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gptkbp:bfsLayer
|
8
|