Statements (51)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
biological pathway
genome editing technology |
| gptkbp:alternativeTo |
gptkb:TALEN
gptkb:ZFN |
| gptkbp:application |
genetic engineering
gene therapy disease modeling functional genomics crop improvement |
| gptkbp:component |
gptkb:CRISPR
gptkb:Cas9_protein |
| gptkbp:discoveredBy |
gptkb:Emmanuelle_Charpentier
gptkb:Jennifer_Doudna |
| gptkbp:discoveredIn |
2012
|
| gptkbp:function |
gene editing
gene knockout gene insertion |
| https://www.w3.org/2000/01/rdf-schema#label |
CRISPR-Cas9 system
|
| gptkbp:limitation |
ethical concerns
delivery challenges off-target effects |
| gptkbp:mechanismOfAction |
RNA-guided DNA cleavage
|
| gptkbp:NobelPrizeYear |
gptkb:2020_Nobel_Prize_in_Chemistry
|
| gptkbp:origin |
bacterial immune system
|
| gptkbp:patentDispute |
gptkb:Broad_Institute
gptkb:University_of_California |
| gptkbp:relatedTo |
gptkb:gene_drive
gptkb:CRISPR gptkb:Cas9 base editing prime editing |
| gptkbp:requires |
gptkb:guide_RNA
PAM sequence |
| gptkbp:target |
specific DNA sequences
|
| gptkbp:usedFor |
animal model creation
epigenome editing plant genome editing transcriptional regulation microbial genome editing disease gene correction functional gene studies genome-wide screening human cell editing knock-in mutations knockout mutations |
| gptkbp:usedIn |
gptkb:biotechnology
gptkb:medicine agriculture |
| gptkbp:bfsParent |
gptkb:guide_RNA
gptkb:guide_RNA_(gRNA) |
| gptkbp:bfsLayer |
6
|