|
gptkbp:instanceOf
|
gptkb:biological_pathway
gptkb:genome_editing_technology
|
|
gptkbp:alternativeTo
|
gptkb:TALEN
gptkb:ZFN
|
|
gptkbp:application
|
gptkb:gene_therapy
genetic engineering
disease modeling
functional genomics
crop improvement
|
|
gptkbp:component
|
gptkb:CRISPR
gptkb:Cas9_protein
|
|
gptkbp:discoveredBy
|
gptkb:Emmanuelle_Charpentier
gptkb:Jennifer_Doudna
|
|
gptkbp:discoveredIn
|
2012
|
|
gptkbp:function
|
gene editing
gene knockout
gene insertion
|
|
gptkbp:limitation
|
ethical concerns
delivery challenges
off-target effects
|
|
gptkbp:mechanismOfAction
|
RNA-guided DNA cleavage
|
|
gptkbp:NobelPrizeYear
|
gptkb:2020_Nobel_Prize_in_Chemistry
|
|
gptkbp:origin
|
gptkb:bacterial_immune_system
|
|
gptkbp:patentDispute
|
gptkb:Broad_Institute
gptkb:University_of_California
|
|
gptkbp:relatedTo
|
gptkb:gene_drive
gptkb:CRISPR
gptkb:Cas9
base editing
prime editing
|
|
gptkbp:requires
|
gptkb:guide_RNA
PAM sequence
|
|
gptkbp:target
|
specific DNA sequences
|
|
gptkbp:usedFor
|
animal model creation
epigenome editing
plant genome editing
transcriptional regulation
microbial genome editing
disease gene correction
functional gene studies
genome-wide screening
human cell editing
knock-in mutations
knockout mutations
|
|
gptkbp:usedIn
|
gptkb:biotechnology
gptkb:medicine
agriculture
|
|
gptkbp:bfsParent
|
gptkb:guide_RNA
|
|
gptkbp:bfsLayer
|
6
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
CRISPR-Cas9 system
|