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gptkbp:instanceOf
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CRISPR-Cas system subtype
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gptkbp:alternativeName
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gptkb:Cpf1_system
gptkb:Cas12_system
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gptkbp:application
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diagnostics
gene knockout
gene insertion
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gptkbp:cleavage
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staggered DNA double-strand breaks
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gptkbp:component
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gptkb:Cas12_protein
gptkb:crRNA
gptkb:tracrRNA
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gptkbp:discoveredBy
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gptkb:Feng_Zhang
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gptkbp:firstDescribed
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2015
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gptkbp:foundIn
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gptkb:Bacteria
archaea
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gptkbp:function
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genome editing
adaptive immunity
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https://www.w3.org/2000/01/rdf-schema#label
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Type V CRISPR-Cas system
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gptkbp:mechanismOfAction
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RNA-guided DNA endonuclease activity
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gptkbp:notableFeature
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single RNA-guided endonuclease
does not require tracrRNA in some subtypes
produces sticky ends
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gptkbp:PAM_requirement
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TTTV
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gptkbp:relatedTo
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gptkb:Type_II_CRISPR-Cas_system
gptkb:Type_VI_CRISPR-Cas_system
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gptkbp:signatureProtein
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gptkb:Cpf1
gptkb:Cas12
gptkb:C2c3
C2c1
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gptkbp:subspecies
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gptkb:V-A
V-B
V-C
V-D
V-E
V-F
V-G
V-H
V-I
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gptkbp:usedIn
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gptkb:DETECTR_diagnostic_platform
gptkb:SHERLOCK_diagnostic_platform
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gptkbp:bfsParent
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gptkb:Cas12k
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gptkbp:bfsLayer
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7
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