Statements (29)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
genome editing system
|
| gptkbp:alsoKnownAs |
CRISPR-Cpf1
Cas12a system |
| gptkbp:cleavage |
double-stranded DNA
|
| gptkbp:component |
gptkb:CRISPR-Cas_systems
|
| gptkbp:contrastsWith |
gptkb:CRISPR-Cas9_system
|
| gptkbp:discoveredBy |
gptkb:Feng_Zhang
|
| gptkbp:discoveredIn |
2015
|
| gptkbp:distinctFrom |
Cpf1 creates staggered DNA cuts
Cpf1 is smaller than Cas9 Cpf1 recognizes T-rich PAM Cpf1 requires only crRNA, not tracrRNA |
| gptkbp:hasApplication |
gptkb:biotechnology
gptkb:medicine agriculture |
| https://www.w3.org/2000/01/rdf-schema#label |
Cpf1 system
|
| gptkbp:patent |
gptkb:Broad_Institute
|
| gptkbp:produces |
sticky ends
|
| gptkbp:recognizesPAM |
TTTV
|
| gptkbp:usedFor |
genome editing
gene knockout gene insertion |
| gptkbp:usedIn |
gptkb:Bacteria
archaea eukaryotic cells |
| gptkbp:usesGuideRNA |
gptkb:crRNA
|
| gptkbp:usesProtein |
gptkb:Cpf1
|
| gptkbp:bfsParent |
gptkb:CRISPR-Cas12_system
|
| gptkbp:bfsLayer |
7
|