Statements (26)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
RNA molecule
|
| gptkbp:binds |
gptkb:Cas9_protein
target DNA |
| gptkbp:category |
biotechnology tool
synthetic RNA |
| gptkbp:composedOf |
gptkb:crRNA
gptkb:tracrRNA |
| gptkbp:designedBy |
gptkb:Emmanuelle_Charpentier
gptkb:Jennifer_Doudna |
| gptkbp:enables |
site-specific genome editing
|
| gptkbp:firstDescribed |
2012
|
| gptkbp:function |
guides Cas9 to target DNA sequence
|
| https://www.w3.org/2000/01/rdf-schema#label |
single guide RNA (sgRNA)
|
| gptkbp:length |
~100 nucleotides
|
| gptkbp:requires |
PAM sequence in target DNA
|
| gptkbp:sequenceSpecificity |
determined by spacer region
|
| gptkbp:targetRecognition |
20-nucleotide guide sequence
|
| gptkbp:usedFor |
genome editing
base editing epigenome editing gene activation gene knockout gene repression |
| gptkbp:usedIn |
gptkb:CRISPR-Cas9_system
|
| gptkbp:bfsParent |
gptkb:guide_RNA_(gRNA)
|
| gptkbp:bfsLayer |
6
|