Statements (53)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
gptkb:protein
endonuclease |
| gptkbp:alternativeName |
gptkb:CRISPR-associated_protein_9
|
| gptkbp:component |
gptkb:CRISPR-Cas_system
|
| gptkbp:discoveredBy |
gptkb:Emmanuelle_Charpentier
gptkb:Jennifer_Doudna |
| gptkbp:EC_number |
3.1.-.-
|
| gptkbp:foundIn |
gptkb:Streptococcus_pyogenes
|
| gptkbp:function |
genome editing
DNA cleavage |
| gptkbp:gene |
cas9
|
| https://www.w3.org/2000/01/rdf-schema#label |
Cas9 endonuclease
|
| gptkbp:locatedOnChromosome |
gptkb:S._pyogenes_genome
|
| gptkbp:mechanismOfAction |
induces double-strand breaks in DNA
|
| gptkbp:molecularWeight |
160 kDa
|
| gptkbp:PAM_sequence |
gptkb:NGG
|
| gptkbp:PDB |
gptkb:4CMP
|
| gptkbp:recognizedBy |
gptkb:guide_RNA
|
| gptkbp:requires |
PAM sequence
Mg2+ |
| gptkbp:UniProtID |
Q99ZW2
|
| gptkbp:usedIn |
gptkb:CRISPR-Cas9_technology
gptkb:agricultural_machinery synthetic biology genome engineering base editing gene therapy prime editing disease modeling functional genomics epigenome editing gene activation gene knock-in gene knockout gene repression high-throughput screening plant genome editing human cell genome editing transcriptional regulation chromatin remodeling RNA targeting (with modifications) animal model generation antiviral research diagnostics (CRISPR-based) in vitro genome editing in vivo genome editing live-cell imaging microbial genome editing multiplex genome editing targeted mutagenesis |
| gptkbp:bfsParent |
gptkb:CRISPR/Cas9
gptkb:CRISPR-Cas9_technology |
| gptkbp:bfsLayer |
6
|