Polymerase Chain Reaction (PCR)
GPTKB entity
Statements (48)
| Predicate | Object |
|---|---|
| gptkbp:instance_of |
gptkb:Biology
|
| gptkbp:can_be_quantified_by |
fluorescent dyes
gel electrophoresis real-time PCR |
| gptkbp:can_create |
millions of copies of DNA
|
| gptkbp:developed_by |
gptkb:Kary_Mullis
|
| gptkbp:first_introduced |
gptkb:1983
|
| gptkbp:has_limitations |
reaction conditions
primer specificity template quality |
| https://www.w3.org/2000/01/rdf-schema#label |
Polymerase Chain Reaction (PCR)
|
| gptkbp:involves |
gptkb:cooperative
annealing denaturation |
| gptkbp:is_essential_for |
gene expression analysis
pathogen detection mutation detection DNA cloning |
| gptkbp:is_part_of |
clinical laboratories
molecular diagnostics biomedical research genetic engineering genomics |
| gptkbp:is_used_in |
gptkb:biotechnology
environmental monitoring cancer research food safety testing vaccine development paternity testing COVID-19 testing HIV testing |
| gptkbp:modifications |
multiplex PCR
nested PCR quantitative PCR (q PCR) reverse transcription PCR (RT-PCR) |
| gptkbp:performed_in |
gptkb:Cooler
|
| gptkbp:requires |
gptkb:RNA_Polymerase
nucleotides DNA template primers |
| gptkbp:sensitivity |
contamination
|
| gptkbp:used_for |
amplifying DNA
|
| gptkbp:used_in |
gptkb:forensic_science
gptkb:research genetic testing medical diagnostics |
| gptkbp:bfsParent |
gptkb:Kary_Mullis
|
| gptkbp:bfsLayer |
4
|