Polymerase Chain Reaction (PCR)
GPTKB entity
Statements (50)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:abbreviation |
PCR
|
| gptkbp:cycleCount |
typically 20-40 cycles
|
| gptkbp:enables |
DNA fingerprinting
cloning of genes detection of pathogens quantification of DNA mutation analysis |
| gptkbp:firstPublished |
Science journal, 1985
|
| https://www.w3.org/2000/01/rdf-schema#label |
Polymerase Chain Reaction (PCR)
|
| gptkbp:introducedIn |
1983
|
| gptkbp:inventedBy |
gptkb:Kary_Mullis
|
| gptkbp:limitation |
contamination risk
inhibitors in samples primer design sensitivity |
| gptkbp:mainEnzyme |
gptkb:Taq_polymerase
|
| gptkbp:NobelPrizeYear |
Kary Mullis received Nobel Prize in Chemistry 1993
|
| gptkbp:patentExpired |
2005
|
| gptkbp:product |
amplified DNA fragments
|
| gptkbp:relatedTo |
gptkb:Sanger_sequencing
molecular diagnostics DNA replication genotyping |
| gptkbp:requires |
nucleotides
DNA polymerase DNA template buffer solution primers thermal cycler |
| gptkbp:step |
annealing
extension denaturation |
| gptkbp:temperature |
~50°C to 98°C
|
| gptkbp:usedFor |
COVID-19 testing
food safety testing paternity testing amplification of DNA ancient DNA studies disease gene detection environmental DNA analysis |
| gptkbp:usedIn |
forensics
genetic research medical diagnostics molecular cloning |
| gptkbp:variant |
gptkb:digital_PCR
gptkb:multiplex_PCR gptkb:real-time_PCR gptkb:reverse_transcription_PCR |
| gptkbp:bfsParent |
gptkb:Cetus_Corporation
|
| gptkbp:bfsLayer |
5
|