PCR (polymerase chain reaction)
GPTKB entity
Statements (52)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:cycleCount |
typically 20-40 cycles
|
| gptkbp:developedBy |
gptkb:Kary_Mullis
|
| gptkbp:enables |
quantification of DNA
detection of specific DNA sequences |
| gptkbp:firstPublished |
Science journal, 1985
|
| gptkbp:hasBiologicalActivity |
gptkb:Taq_polymerase
|
| https://www.w3.org/2000/01/rdf-schema#label |
PCR (polymerase chain reaction)
|
| gptkbp:impact |
revolutionized molecular biology
|
| gptkbp:introducedIn |
1983
|
| gptkbp:NobelPrizeYear |
Kary Mullis received Nobel Prize in Chemistry 1993
|
| gptkbp:patent |
gptkb:US4683195A
|
| gptkbp:requires |
nucleotides
DNA polymerase DNA template buffer solution primers |
| gptkbp:step |
annealing
extension denaturation |
| gptkbp:usedFor |
gptkb:genetic_fingerprinting
COVID-19 testing food safety testing gene expression analysis paternity testing biodiversity studies phylogenetic analysis cancer diagnostics amplification of DNA mutation detection microbial identification epigenetic studies ancient DNA analysis pathogen detection HIV detection GMO detection cloning DNA fragments environmental DNA studies gene mapping prenatal genetic diagnosis |
| gptkbp:usedIn |
forensic science
genetic research medical diagnostics molecular cloning disease detection |
| gptkbp:variant |
gptkb:digital_PCR
gptkb:multiplex_PCR gptkb:real-time_PCR gptkb:reverse_transcription_PCR |
| gptkbp:bfsParent |
gptkb:FilmArray
gptkb:Pyrococcus_furiosus |
| gptkbp:bfsLayer |
6
|