CRISPR interference (CRISPRi)

URI: https://gptkb.org/entity/CRISPR_interference_(CRISPRi)
GPTKB entity
Statements (30)
Predicate Object
gptkbp:instanceOf gptkb:gene_regulation_method
gptkb:genome_editing_technique
gptkbp:advantage high specificity
multiplexing capability
reversible gene regulation
gptkbp:application synthetic biology
functional genomics
gene knockdown
gptkbp:basedOn gptkb:CRISPR-Cas_system
gptkbp:combines epigenetic modifiers
gptkbp:developedBy gptkb:Jennifer_Doudna
gptkb:Stanley_Qi
gptkb:Wendell_Lim
gptkbp:doesNotRegulate cut DNA
gptkbp:effect transcriptional repression
gptkbp:firstDescribed 2013
gptkbp:function represses gene expression
gptkbp:mechanismOfAction blocks transcription elongation
blocks transcription initiation
gptkbp:organism gptkb:Bacteria
eukaryotes
gptkbp:relatedTo gptkb:CRISPR_activation_(CRISPRa)
RNA interference (RNAi)
gptkbp:target coding sequence
promoter region
gptkbp:uses gptkb:single_guide_RNA_(sgRNA)
gptkb:catalytically_dead_Cas9_(dCas9)
gptkbp:bfsParent gptkb:guide_RNA_(gRNA)
gptkbp:bfsLayer 7
https://www.w3.org/2000/01/rdf-schema#label CRISPR interference (CRISPRi)