CRISPR interference (CRISPRi)
GPTKB entity
Statements (30)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
gene regulation method
genome editing technique |
| gptkbp:advantage |
high specificity
multiplexing capability reversible gene regulation |
| gptkbp:application |
synthetic biology
functional genomics gene knockdown |
| gptkbp:basedOn |
gptkb:CRISPR-Cas_system
|
| gptkbp:combines |
epigenetic modifiers
|
| gptkbp:developedBy |
gptkb:Jennifer_Doudna
gptkb:Stanley_Qi gptkb:Wendell_Lim |
| gptkbp:doesNotRegulate |
cut DNA
|
| gptkbp:effect |
transcriptional repression
|
| gptkbp:firstDescribed |
2013
|
| gptkbp:function |
represses gene expression
|
| https://www.w3.org/2000/01/rdf-schema#label |
CRISPR interference (CRISPRi)
|
| gptkbp:mechanismOfAction |
blocks transcription elongation
blocks transcription initiation |
| gptkbp:organism |
gptkb:Bacteria
eukaryotes |
| gptkbp:relatedTo |
gptkb:CRISPR_activation_(CRISPRa)
RNA interference (RNAi) |
| gptkbp:target |
coding sequence
promoter region |
| gptkbp:uses |
gptkb:single_guide_RNA_(sgRNA)
gptkb:catalytically_dead_Cas9_(dCas9) |
| gptkbp:bfsParent |
gptkb:guide_RNA_(gRNA)
|
| gptkbp:bfsLayer |
6
|