catalytically dead Cas9 (dCas9)
GPTKB entity
Statements (24)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
engineered protein
genome engineering tool |
| gptkbp:alternativeName |
dead Cas9
nuclease-deficient Cas9 |
| gptkbp:derivedFrom |
gptkb:CRISPR-associated_protein_9_(Cas9)
|
| gptkbp:excludes |
endonuclease activity
|
| gptkbp:firstDescribed |
2013
|
| gptkbp:function |
binds DNA without cutting
|
| https://www.w3.org/2000/01/rdf-schema#label |
catalytically dead Cas9 (dCas9)
|
| gptkbp:mutationAssociatedWith |
D10A
H840A |
| gptkbp:requires |
gptkb:guide_RNA
|
| gptkbp:usedFor |
transcriptional activation
gene regulation epigenome editing transcriptional repression |
| gptkbp:usedIn |
gptkb:CRISPR_activation_(CRISPRa)
gptkb:CRISPR_interference_(CRISPRi) synthetic biology functional genomics |
| gptkbp:bfsParent |
gptkb:CRISPR_activation_(CRISPRa)
gptkb:CRISPR_interference_(CRISPRi) gptkb:CRISPR_activation |
| gptkbp:bfsLayer |
7
|