CRISPR activation (CRISPRa)

URI: https://gptkb.org/entity/CRISPR_activation_(CRISPRa)
GPTKB entity
Statements (32)
Predicate Object
gptkbp:instanceOf genome engineering technique
gptkbp:activatorDomainExamples gptkb:Rta
VP64
p65
gptkbp:alsoKnownAs gptkb:CRISPRa
gptkbp:application cell reprogramming
disease modeling
functional genomics
gene expression studies
gptkbp:basedOn gptkb:CRISPR-Cas9_system
gptkbp:deliveredBy viral vectors
plasmid transfection
electroporation
gptkbp:differenceFromCRISPRi activates rather than represses gene expression
gptkbp:doesNotRegulate edit DNA sequence
gptkbp:enables multiplex gene activation
reversible gene activation
targeted gene upregulation
gptkbp:firstDescribed 2015
gptkbp:function transcriptional activation
gene activation
https://www.w3.org/2000/01/rdf-schema#label CRISPR activation (CRISPRa)
gptkbp:organismUsedIn gptkb:yeast
plants
human cells
mouse cells
gptkbp:relatedTo gptkb:CRISPR_interference_(CRISPRi)
gptkbp:uses gptkb:guide_RNA_(gRNA)
gptkb:catalytically_dead_Cas9_(dCas9)
transcriptional activator domains
gptkbp:bfsParent gptkb:guide_RNA_(gRNA)
gptkbp:bfsLayer 6