Statements (51)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
genome editing technology
|
| gptkbp:activatorDomainExamples |
gptkb:Rta
VP64 p65 |
| gptkbp:alternativeName |
CRISPR-mediated gene activation
CRISPR-on |
| gptkbp:application |
drug discovery
synthetic biology disease modeling gene therapy research |
| gptkbp:component |
gptkb:guide_RNA
transcriptional activator domain dCas9 protein |
| gptkbp:deliveredBy |
viral vectors
plasmid transfection mRNA delivery |
| gptkbp:developedBy |
2015
|
| gptkbp:distinctFrom |
CRISPR knockout
CRISPRi |
| gptkbp:doesNotRegulate |
edit DNA sequence
|
| gptkbp:effect |
upregulates target gene expression
|
| gptkbp:fullName |
gptkb:CRISPR_activation
|
| gptkbp:function |
gene activation
|
| https://www.w3.org/2000/01/rdf-schema#label |
CRISPRa
|
| gptkbp:limitation |
delivery challenges
off-target effects variable activation efficiency |
| gptkbp:mechanismOfAction |
fused to transcriptional activators
uses catalytically dead Cas9 (dCas9) |
| gptkbp:notableContributor |
gptkb:Feng_Zhang
gptkb:George_Church gptkb:Stanley_Qi |
| gptkbp:patent |
gptkb:Broad_Institute
gptkb:Stanford_University |
| gptkbp:publishedIn |
Cell 2013, Gilbert et al.
Cell 2013, Mali et al. Cell 2015, Chavez et al. |
| gptkbp:regulates |
endogenous genes
exogenous genes |
| gptkbp:relatedTo |
gptkb:CRISPR
gptkb:CRISPR-Cas9 |
| gptkbp:species |
gptkb:yeast
plants human cells mouse cells |
| gptkbp:usedIn |
cell reprogramming
functional genomics gene expression studies |
| gptkbp:bfsParent |
gptkb:CRISPR_activation_(CRISPRa)
gptkb:CRISPR_activation |
| gptkbp:bfsLayer |
7
|