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gptkbp:instanceOf
|
genome editing technology
bacterial immune system
|
|
gptkbp:advantage
|
collateral cleavage activity
produces staggered DNA cuts
simpler guide RNA structure
|
|
gptkbp:alternativeName
|
gptkb:Cpf1_system
|
|
gptkbp:application
|
molecular diagnostics
gene activation
gene knockout
gene repression
|
|
gptkbp:component
|
CRISPR adaptive immune system
|
|
gptkbp:contains
|
gptkb:CRISPR_RNA_(crRNA)
gptkb:Cas12_protein
|
|
gptkbp:discoveredBy
|
gptkb:Feng_Zhang
|
|
gptkbp:family
|
gptkb:Class_2_CRISPR_systems
|
|
gptkbp:firstDescribed
|
2015
|
|
gptkbp:foundIn
|
gptkb:Bacteria
archaea
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
CRISPR-Cas12 system
|
|
gptkbp:limitation
|
off-target effects
PAM sequence requirement
|
|
gptkbp:mechanismOfAction
|
targeted DNA cleavage
collateral single-stranded DNA cleavage
|
|
gptkbp:notableVariant
|
gptkb:Cas12a
gptkb:Cas12b
gptkb:Cas12c
gptkb:Cas12e
gptkb:Cas12f
gptkb:Cas12g
|
|
gptkbp:PAMSequence
|
TTTV (where V is A, C, or G)
|
|
gptkbp:patent
|
gptkb:Broad_Institute
gptkb:Harvard_University
gptkb:MIT
|
|
gptkbp:recognizedBy
|
PAM sequence
|
|
gptkbp:regulates
|
gptkb:crRNA
tracrRNA (in some variants)
|
|
gptkbp:relatedTo
|
gptkb:CRISPR-Cas9_system
|
|
gptkbp:subspecies
|
gptkb:Type_V_CRISPR_system
|
|
gptkbp:target
|
double-stranded DNA
|
|
gptkbp:usedFor
|
genome editing
nucleic acid detection
|
|
gptkbp:usedIn
|
gptkb:DETECTR_diagnostic_platform
gptkb:SHERLOCK_diagnostic_platform
point-of-care testing
COVID-19 diagnostics
animal genome editing
plant genome editing
human genome editing research
|
|
gptkbp:bfsParent
|
gptkb:Cas12_protein
|
|
gptkbp:bfsLayer
|
6
|