Statements (53)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:alsoKnownAs |
gptkb:real-time_PCR
qPCR |
| gptkbp:analyzes |
amplification curves
|
| gptkbp:appliesTo |
gptkb:agricultural_machinery
forensic science environmental monitoring clinical diagnostics |
| gptkbp:canBe |
gptkb:digital_PCR
gptkb:TaqMan_probes real-time analysis Molecular Beacons SYBR Green Scorpion probes endpoint analysis multiplex qPCR one-step qPCR reverse transcription qPCR singleplex qPCR two-step qPCR |
| gptkbp:detects |
single nucleotide polymorphisms
copy number variations viral load bacterial load |
| gptkbp:developedBy |
1990s
|
| gptkbp:enables |
absolute quantification
high-throughput analysis relative quantification |
| https://www.w3.org/2000/01/rdf-schema#label |
quantitative PCR
|
| gptkbp:measures |
genomic DNA
cDNA RNA (via RT-qPCR) amplification of DNA in real time |
| gptkbp:monitors |
fluorescence intensity
|
| gptkbp:relatedTo |
gptkb:polymerase_chain_reaction
|
| gptkbp:requires |
gptkb:dNTPs
DNA polymerase buffer solution primers thermal cycler with fluorescence detection template DNA or RNA |
| gptkbp:sensitivity |
contamination
primer-dimer formation |
| gptkbp:usedFor |
gene expression analysis
detection of pathogens quantification of nucleic acids |
| gptkbp:uses |
fluorescent dyes
sequence-specific DNA probes |
| gptkbp:bfsParent |
gptkb:reverse_transcription_polymerase_chain_reaction
gptkb:digital_PCR gptkb:real-time_PCR gptkb:reverse_transcription_PCR |
| gptkbp:bfsLayer |
6
|