Statements (27)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:application |
cloning
genotyping mutation detection pathogen detection |
| gptkbp:contrastsWith |
gptkb:real-time_PCR
|
| gptkbp:detects |
gel electrophoresis
|
| gptkbp:developedBy |
gptkb:Kary_Mullis
|
| gptkbp:fullName |
conventional polymerase chain reaction
|
| https://www.w3.org/2000/01/rdf-schema#label |
conventional PCR
|
| gptkbp:introducedIn |
1980s
|
| gptkbp:limitation |
lower sensitivity than qPCR
not quantitative |
| gptkbp:output |
end-point analysis
|
| gptkbp:requires |
nucleotides
DNA polymerase DNA template buffer solution primers thermal cycler |
| gptkbp:step |
annealing
extension denaturation |
| gptkbp:usedFor |
DNA amplification
|
| gptkbp:bfsParent |
gptkb:multiplex_PCR
gptkb:real-time_PCR |
| gptkbp:bfsLayer |
6
|