TaqMan probes

GPTKB entity

Statements (23)
Predicate Object
gptkbp:instanceOf molecular biology technique
gptkbp:alternativeTo Molecular Beacons
SYBR Green
gptkbp:application gene expression analysis
genotyping
pathogen detection
gptkbp:contains fluorescent reporter dye
quencher dye
gptkbp:detects specific DNA sequences
amplification products in real-time
gptkbp:firstDescribed 1996
https://www.w3.org/2000/01/rdf-schema#label TaqMan probes
gptkbp:inventedBy gptkb:Applied_Biosystems
gptkbp:mechanismOfAction 5' nuclease activity of Taq polymerase
gptkbp:patent gptkb:Roche_Molecular_Systems
gptkbp:relatedTo qPCR
hydrolysis probes
gptkbp:requires thermal cycler with fluorescence detection
gptkbp:usedFor gptkb:quantitative_PCR
gptkbp:usedIn gptkb:real-time_PCR
gptkbp:bfsParent gptkb:quantitative_PCR
gptkb:LightCycler
gptkbp:bfsLayer 7