Statements (48)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
gptkb:gene
|
| gptkbp:discoveredBy |
gptkb:François_Jacob
gptkb:Jacques_Monod |
| gptkbp:EC_number |
3.2.1.23
|
| gptkbp:encodes |
gptkb:beta-galactosidase
|
| gptkbp:Entrez_Gene_ID |
945006
|
| gptkbp:enzyme |
beta-galactosidase activity
|
| gptkbp:firstDescribed |
1960
|
| gptkbp:foundIn |
gptkb:Bacteria
|
| gptkbp:gene |
gptkb:lacZ
|
| https://www.w3.org/2000/01/rdf-schema#label |
lacZ gene (in E. coli)
|
| gptkbp:locatedOnChromosome |
gptkb:E._coli_chromosome
|
| gptkbp:molecularWeight |
116 kDa (for beta-galactosidase monomer)
|
| gptkbp:mutationAssociatedWith |
loss of lactose metabolism
|
| gptkbp:orthologIn |
other enterobacteria
|
| gptkbp:partOf |
gptkb:lac_operon
|
| gptkbp:product |
glucose
galactose tetramer beta-galactosidase (LacZ protein) blue pigment (with X-gal) |
| gptkbp:productFunction |
hydrolyzes lactose into glucose and galactose
|
| gptkbp:regulates |
induced by lactose
repressed by glucose affected by CAP-cAMP complex controlled by lac promoter repressed by lac repressor |
| gptkbp:sequence |
3072 base pairs
|
| gptkbp:substrate |
gptkb:X-gal
lactose |
| gptkbp:usedAs |
reporter gene
|
| gptkbp:usedIn |
gptkb:blue-white_screening
biosensors synthetic biology genetic engineering molecular cloning gene expression studies enzyme assays cell lineage tracing histochemical staining bacterial two-hybrid systems complementation assays reporter assays in eukaryotes transcriptional fusion assays transgenic animal studies translational fusion assays |
| gptkbp:bfsParent |
gptkb:Beta-galactosidase
|
| gptkbp:bfsLayer |
7
|