Statements (25)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:color_of_non-recombinant_colonies |
blue
|
| gptkbp:color_of_recombinant_colonies |
white
|
| gptkbp:common_host_strain |
E. coli DH5α
|
| gptkbp:common_vector |
pUC19
|
| gptkbp:detects |
disruption of lacZ gene
|
| gptkbp:developedBy |
1970s
|
| https://www.w3.org/2000/01/rdf-schema#label |
blue-white screening
|
| gptkbp:inducedBy |
IPTG
|
| gptkbp:involves |
α-complementation
|
| gptkbp:produces |
blue colonies (non-recombinant)
white colonies (recombinant) |
| gptkbp:relies_on |
IPTG inducer
X-gal substrate lacZ gene |
| gptkbp:requires |
E. coli host
plasmid vector with lacZα fragment |
| gptkbp:substrate |
gptkb:X-gal
|
| gptkbp:used_in |
cloning
plasmid selection |
| gptkbp:usedFor |
identification of recombinant bacteria
|
| gptkbp:bfsParent |
gptkb:beta-galactosidase
gptkb:lacZ gptkb:Beta-galactosidase |
| gptkbp:bfsLayer |
7
|