|
gptkbp:instanceOf
|
gptkb:enzyme
|
|
gptkbp:CASNumber
|
gptkb:9031-11-2
|
|
gptkbp:catalyzes
|
hydrolysis of beta-galactosides into monosaccharides
|
|
gptkbp:deficiencyCauses
|
gptkb:GM1_gangliosidosis_(in_humans)
|
|
gptkbp:discoveredBy
|
gptkb:François_Jacob
gptkb:Jacques_Monod
|
|
gptkbp:EC_number
|
3.2.1.23
|
|
gptkbp:encodedBy
|
gptkb:lacZ_gene_(in_E._coli)
|
|
gptkbp:foundIn
|
gptkb:Bacteria
humans
other organisms
|
|
gptkbp:GO_term
|
gptkb:GO:0004565
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
Beta-galactosidase
|
|
gptkbp:inhibitedBy
|
gptkb:isopropyl_β-D-1-thiogalactopyranoside_(IPTG)
|
|
gptkbp:molecularWeight
|
approximately 465 kDa (E. coli)
|
|
gptkbp:Nobel_Prize_related
|
gptkb:1965_Nobel_Prize_in_Physiology_or_Medicine_(Monod,_Jacob,_Lwoff)
|
|
gptkbp:optimum_pH
|
37°C (E. coli)
6.5–7.5 (E. coli)
|
|
gptkbp:PDB
|
gptkb:1DP0
|
|
gptkbp:product
|
glucose
galactose
5-bromo-4-chloro-indoxyl (from X-gal)
o-nitrophenol (from ONPG)
|
|
gptkbp:substrate
|
gptkb:ONPG
gptkb:X-gal
lactose
|
|
gptkbp:subunit
|
tetramer (E. coli)
|
|
gptkbp:systematicName
|
gptkb:beta-D-galactoside_galactohydrolase
|
|
gptkbp:UniProtID
|
gptkb:P00722_(E._coli)
|
|
gptkbp:used_in
|
gptkb:blue-white_screening
|
|
gptkbp:usedFor
|
reporter gene
|
|
gptkbp:bfsParent
|
gptkb:Enzymes
|
|
gptkbp:bfsLayer
|
6
|