Statements (35)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:abbreviation |
gptkb:ddPCR
|
| gptkbp:advantage |
high sensitivity
absolute quantification without standard curve high precision |
| gptkbp:application |
gene expression analysis
liquid biopsy GMO detection copy number variation analysis rare mutation detection viral load quantification |
| gptkbp:basedOn |
gptkb:polymerase_chain_reaction
|
| gptkbp:contrastsWith |
gptkb:quantitative_PCR
|
| gptkbp:detects |
fluorescence
|
| gptkbp:developedBy |
gptkb:Bio-Rad_Laboratories
|
| gptkbp:distinctFrom |
qPCR uses standard curves, ddPCR does not
|
| gptkbp:dividesSampleInto |
thousands of nanoliter-sized droplets
|
| gptkbp:firstDescribed |
2011
|
| https://www.w3.org/2000/01/rdf-schema#label |
droplet digital PCR
|
| gptkbp:limitation |
higher cost than qPCR
lower throughput than qPCR |
| gptkbp:marketedAs |
gptkb:Bio-Rad_Laboratories
gptkb:RainDance_Technologies |
| gptkbp:relatedTo |
gptkb:digital_PCR
microfluidics next-generation sequencing |
| gptkbp:requires |
thermal cycler
droplet generator droplet reader fluorescent probes or dyes |
| gptkbp:usedFor |
absolute quantification of DNA
absolute quantification of RNA quantitative nucleic acid analysis |
| gptkbp:bfsParent |
gptkb:digital_PCR
|
| gptkbp:bfsLayer |
6
|