Statements (36)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
genome editing technology
|
| gptkbp:advantage |
high specificity
|
| gptkbp:application |
animal genome editing
plant genome editing human cell genome editing |
| gptkbp:contrastsWith |
CRISPR genome editing
ZFN genome editing |
| gptkbp:deliveredBy |
viral vectors
mRNA injection plasmid transfection |
| gptkbp:developedBy |
gptkb:Joung_Lab
gptkb:Voytas_Lab |
| gptkbp:enables |
gene knockout
gene correction gene insertion targeted DNA double-strand breaks |
| gptkbp:firstPublished |
2010
|
| gptkbp:fullName |
Transcription Activator-Like Effector Nuclease genome editing
|
| https://www.w3.org/2000/01/rdf-schema#label |
TALEN genome editing
|
| gptkbp:limitation |
costly protein engineering
labor-intensive design |
| gptkbp:mechanismOfAction |
engineered DNA-binding domain fused to FokI nuclease
|
| gptkbp:nucleaseDomain |
gptkb:FokI
|
| gptkbp:patent |
gptkb:Sangamo_BioSciences
|
| gptkbp:recognizedBy |
modular DNA-binding repeats
|
| gptkbp:requires |
dimerization for DNA cleavage
|
| gptkbp:target |
specific DNA sequences
|
| gptkbp:usedFor |
crop improvement
therapeutic development gene function studies |
| gptkbp:usedIn |
disease modeling
functional genomics gene therapy research |
| gptkbp:uses |
gptkb:TALENs
|
| gptkbp:bfsParent |
gptkb:CRISPR-Cas12_genome_editing_technology
|
| gptkbp:bfsLayer |
5
|