|
gptkbp:instanceOf
|
gptkb:protein
endonuclease
|
|
gptkbp:alternativeTo
|
gptkb:Cpf1
gptkb:AsCas12a
SaCas9
|
|
gptkbp:application
|
gptkb:biotechnology
gptkb:medicine
agriculture
|
|
gptkbp:containsGene
|
cas9
|
|
gptkbp:discoveredBy
|
gptkb:Emmanuelle_Charpentier
gptkb:Jennifer_Doudna
|
|
gptkbp:discoveredIn
|
2012
|
|
gptkbp:domain
|
gptkb:PAM-interacting_domain
gptkb:HNH
gptkb:RuvC
gptkb:CTD
gptkb:REC_lobe
NTD
Bridge helix
|
|
gptkbp:enzymeCommissionNumber
|
EC 3.1.-.-
|
|
gptkbp:fullName
|
gptkb:Streptococcus_pyogenes_Cas9
|
|
gptkbp:function
|
genome editing
DNA cleavage
|
|
gptkbp:hasActivity
|
RNA-guided DNA endonuclease activity
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
SpCas9
|
|
gptkbp:mechanismOfAction
|
induces double-strand breaks
|
|
gptkbp:molecularWeight
|
~160 kDa
|
|
gptkbp:mutationAssociatedWith
|
gptkb:dCas9
SpCas9-HF1
eSpCas9
|
|
gptkbp:organismOfOrigin
|
gptkb:Streptococcus_pyogenes
|
|
gptkbp:PDB
|
gptkb:4CMP
4OO8
|
|
gptkbp:recognizesPAM
|
gptkb:NGG
|
|
gptkbp:requires
|
gptkb:crRNA
gptkb:guide_RNA
gptkb:tracrRNA
|
|
gptkbp:UniProtID
|
Q99ZW2
|
|
gptkbp:usedFor
|
genome engineering
gene knock-in
gene knockout
|
|
gptkbp:usedIn
|
gptkb:CRISPR-Cas9_system
synthetic biology
base editing
gene therapy
prime editing
disease modeling
functional genomics
crop improvement
|
|
gptkbp:bfsParent
|
gptkb:LbCas12a
gptkb:Streptococcus_pyogenes_Cas9
|
|
gptkbp:bfsLayer
|
8
|